抗BRaf(V600E)鼠单克隆抗体
b-raf (v600e) [26039]
[100 µl]
catalog number: 26039
gene symbol: b-raf
description: anti-b-raf(v600e) mouse monoclonal antibody
background: b-raf is a member of the raf family of ser/thr protein kinases. it functions downstream of ras to regulate the map kinase signaling pathway. mutations in the braf gene cause diseases. inherited mutations in braf cause cardiofaciocutaneous syndrome. acquired mutations in braf have been found in cancers
immunogen: a synthetic peptide from the internal region of braf(v600e), human origin.
applications: elisa, wb, ihc, ip
recommended dilutions:
elisa: 1:100-1:5000
wb: 1:100-1:2000
ihc: 1:100-1:1000
concentration: 0.5 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igg2b
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: recognizes b-raf(v600e), but not wild type b-raf proteins from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
western blot:
western blot analysis of recombinant b-raf proteins with anti-braf(v600e) antibody. purified his - tagged b-raf( v600e ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), his - tagged b-raf( v600d ) (lane 3) , his - tagged b-raf( v600a ) (lane 4) , his - tagged b-raf( v600g ) (lane 5) , his - tagged b-raf( v600k ) (lane 6), his - tagged b-raf( v600r ) (lane 7), and his - tagged b-raf( v600m ) (lane 8) were blotted with anti-b-raf(v600e) monoclonal antibody (cat # 26039) .
immunofluorescence:
immunofluorescence of cell expressing braf protein with anti-braf(v600e) antibody . hek293t cells were transfected with pcdna3-gfp- b-raf( v600e )plasmid,pcdna3-gfp- b-raf( wt )plasmid, pcdna3-gfp- b-raf( v600k )plasmid,pcdna3-gfp- b-raf( v600r )plasmid, pcdna3-gfp- b-raf( v600a )plasmid,pcdna3-gfp- b-raf( v600d ) plasmid, pcdna3-gfp-b-raf(v600m)plasmid, or pcdna3-gfp-b-raf(v600g)plasmid, then fixed and stained with anti-b-raf(v600e) monoclonal antibody (cat. # 26039).
immunohistochemistry:
immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti-b-raf (v600e) monoclonal antibody (cat. # 26039). tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °c. antigen retrieval was by heat mediation in citrate buffer (ph6). samples were then incubated with primary antibody (1:100) overnight at 4°c. a hrp-conjugated goat anti-mouse igg (dilution 1:50) was used as secondary antibody.
publications:
1.
mod pathol. 2013 mar;26(3):414-20.
2.
tumor biology. june 2014, volume 35, issue 6, pp 5727-5733
3.
human pathology. february 2015volume 46, issue 2, pages 169–175
4.
human pathology. november 2013volume 44, issue 11, pages 2563–2570
5.
seminars in diagnostic pathology
武汉纽斯特生物技术有限公司
18972339958
中国 武汉
甘肃进口真空吸尘器价格
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登封建筑设计院排名 圣之杰
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抗BRaf(V600E)鼠单克隆抗体
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贵州那里有凉皮培训 全年无忧淡季
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供应低价蝴蝶盖、千秋盖、各种塑料盖
[100 µl]
catalog number: 26039
gene symbol: b-raf
description: anti-b-raf(v600e) mouse monoclonal antibody
background: b-raf is a member of the raf family of ser/thr protein kinases. it functions downstream of ras to regulate the map kinase signaling pathway. mutations in the braf gene cause diseases. inherited mutations in braf cause cardiofaciocutaneous syndrome. acquired mutations in braf have been found in cancers
immunogen: a synthetic peptide from the internal region of braf(v600e), human origin.
applications: elisa, wb, ihc, ip
recommended dilutions:
elisa: 1:100-1:5000
wb: 1:100-1:2000
ihc: 1:100-1:1000
concentration: 0.5 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igg2b
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: recognizes b-raf(v600e), but not wild type b-raf proteins from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
western blot:
western blot analysis of recombinant b-raf proteins with anti-braf(v600e) antibody. purified his - tagged b-raf( v600e ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), his - tagged b-raf( v600d ) (lane 3) , his - tagged b-raf( v600a ) (lane 4) , his - tagged b-raf( v600g ) (lane 5) , his - tagged b-raf( v600k ) (lane 6), his - tagged b-raf( v600r ) (lane 7), and his - tagged b-raf( v600m ) (lane 8) were blotted with anti-b-raf(v600e) monoclonal antibody (cat # 26039) .
immunofluorescence:
immunofluorescence of cell expressing braf protein with anti-braf(v600e) antibody . hek293t cells were transfected with pcdna3-gfp- b-raf( v600e )plasmid,pcdna3-gfp- b-raf( wt )plasmid, pcdna3-gfp- b-raf( v600k )plasmid,pcdna3-gfp- b-raf( v600r )plasmid, pcdna3-gfp- b-raf( v600a )plasmid,pcdna3-gfp- b-raf( v600d ) plasmid, pcdna3-gfp-b-raf(v600m)plasmid, or pcdna3-gfp-b-raf(v600g)plasmid, then fixed and stained with anti-b-raf(v600e) monoclonal antibody (cat. # 26039).
immunohistochemistry:
immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti-b-raf (v600e) monoclonal antibody (cat. # 26039). tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °c. antigen retrieval was by heat mediation in citrate buffer (ph6). samples were then incubated with primary antibody (1:100) overnight at 4°c. a hrp-conjugated goat anti-mouse igg (dilution 1:50) was used as secondary antibody.
publications:
1.
mod pathol. 2013 mar;26(3):414-20.
2.
tumor biology. june 2014, volume 35, issue 6, pp 5727-5733
3.
human pathology. february 2015volume 46, issue 2, pages 169–175
4.
human pathology. november 2013volume 44, issue 11, pages 2563–2570
5.
seminars in diagnostic pathology
武汉纽斯特生物技术有限公司
18972339958
中国 武汉
甘肃进口真空吸尘器价格
一步法黄夹克保温管生产厂家
餐饮店经营五大法则 你知道几条
登封建筑设计院排名 圣之杰
辽宁铝艺生产厂家可批发销售
抗BRaf(V600E)鼠单克隆抗体
裕华区G3云推广网络优化哪家正规帝佑科技
贵州那里有凉皮培训 全年无忧淡季
供应华为局端设备/程控交换机C&C08板件ALM
山东别墅铜铝门价格豪阳门业品质因为天成
厂家直销 欧式折叠收纳袋 购物袋
电动汽车故障多发在出厂第二年夏季 起火车辆19%处于充电状态
供应：光纤适配器，SC,FC，LC，ST光纤适配器
猪拉稀的药，猪拉稀腹泻痢疾特效药
火锅加盟哪个品牌好呢 有拈头主题火锅连锁开店利润高
长白公猪采购，到青盛源，成活率高，免疫力强
童装代理哪个品牌，米奇姆童装带来广阔的发展商机
诸城国邦调味品加工设备
白云互动触摸广告机厂家_品牌供应
供应低价蝴蝶盖、千秋盖、各种塑料盖